lipe hk 2 - HK2 An immortalized proximal tubule epithelial rtp slot cinema777 17 cell line from normal HK2 human kidney 2 is a proximal tubular cell PTC line derived from normal kidney The cells were immortalized by transduction with human papilloma virus 16 HPV16 E6E7 genes The cell line appears to be derived from a single cell based on Southern and FISH analysis Age Cellosaurus HK2 Human kidney CVCL0302 Part of Cancer Dependency Map project DepMap includes Cancer Cell Line Encyclopedia CCLE Population Caucasian Genetic integration MethodTransduction GeneUniProtKB P03126 Human papillomavirus type 16 protein E6 Genetic integration MethodTransduction GeneUniProtKB P03129 Human The doubling time for HK2 cells ranges between 473 h and 617 h Adherent or in Suspension HK2 kidney epithelial cell line is adherent Subcultivation ratio HK2 cells are subcultivated at a ratio of 12 to 14 Adherent HK2 cells are washed with 1 x PBS and incubated at ambient temperature for 8 to 10 minutes after adding Accutase HK2 an immortalized proximal tubule epithelial cell line PubMed A primary PTC culture from normal adult human renal cortex was exposed to a recombinant retrovirus containing the HPV 16 E6E7 genes resulting in a cell line designated HK2 human kidney2 which has grown continuously in serum free media for more than one year HK2 cell growth is epidermal growth factor dependent and the cells retain a PDF HK2 Cell Line Elabscience HK2 cells and LPS treatment The inflammatory response and cell damage of LPSinduced apoptosis in HK2 cells is a cellular model of acute kidney injury AKI which is one of the most fatal inflammatory sepsis Huang et al 2017 Shen et al 2019 Zhang et al 2020Therefore HK2 cells were purchased from BeNa culture collection Cat No BNCC100395 Beijing China and were cultured in Laboratory Investigation HK2 An immortalized proximal tubule epithelial cell line from normal adult human kidney Michael J Ryan 1 Gretchen Johnson 1 Judy Kirk 1 Sally M Fuerstenberg 1 Richard A mimpi ikan tongkol togel 13 Zager 1 Beverly TorokStorb 1 1 The Fred Hutchinson Cancer Research Center FHCRC and the Department of Medicine University of Washington Seattle Washington USA The Fred Hutchinson Cellosaurus cell line HK2 Human kidney CVCL0302 HK2 cells retain functional characteristics of proximal tubular epithelium such as Nadependentphlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid but not to antidiuretic hormone The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen HK2 cells are anchorage In Vitro to In Vivo Concordance of Toxicity Using the Human Proximal The aim of this study was to investigate the expression and function of drug transporters in HK2 cells and their suitability as an in vitro model of the human proximal tubule qPCR showed no mRNA expression of the SLC22 transporter family OAT1 OAT3 OCT2 in HK2 cells compared to renal cortex samples In contrast SLC16A1 MCT1 which is Confluent cultures of HK2 cells a renal epithelial cell line of human origin were exposed to various concentrations of oxalate 02 to 20 mmolL and lipopolysaccharide LPS 01 and 10 μgmL for various time points 424 h under serumfree conditions The conditioned mediums were collected and an IL6 protein level was measured by enzymelinked immunosorbent assay ELISA All stock solutions Tables 1 and 2 were serially diluted in HK2 cell culture media by 10 2 10 3 10 4 10 5 and 10 6fold in cell culture medium and were well solubilized Treated and nontreated HK2 cells were incubated for 24 hours at 37 C in a humidified incubator with 5 CO 2 prior to performing end point assays Assays were HK2 Cells Probing Kidney Function and Pathology with HK2 Cells in The limitations of renal epithelial cell line HK2 as a model of drug LincKIAA17372 promoted LPSinduced HK2 cell apoptosis by regulating Hk2 Crl2190 Atcc Oxalate stimulates IL6 production in HK2 cells a kombo 88 slot login 5 line of human renal
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